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Westing Blotting Services

Apex BioTek offers Western blot custom services including extraction of total protein from tissues or cells , construction of Western blots, immunostaining blots with any antibody, quantitative analysis of immunostaining, and sendiing high quality digital images. 

  • Construction of Western blots:

  • Harvesting fresh tissues/cultured cells provided.

  • Homogenization of tissues/cells in protein lysis buffer supplemented with a cocktail of 7 mammalian protease inhibitors.

  • Quantitation of  total protein 

  • Testing the purity and integrity of protein by gel electrophoresis.

  • Fractionation of protein (75 microgram) through large SDS-denatured polyacrylamide gel electrophoresis.

  • Transfer protein onto PVDF membranes.

  • Shipping blots at room temperature in sealed bags

  • Western Blot Analysis:

  • Immunostaining blots with antibodies provided  using ultrasensative chemiluminescent ECL detection

  • Quantitative analysis of immunostaining

  • Sending high quality digital images of X-ray films.

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